Straightforward method to gain rapid knowledge about the structure Molecules ( 1, 2) obtained from multiple species is a Combining the deposited data about informational Radically changed the traditional approach to gene discovery andĬharacterization. Products (RNAs and proteins), genomes and molecular functions has Large databases containing growing information about genes, gene Since the late 1990s, the availability of public, In this review, we discuss the causes of this issue, its quantitative incidence in biomedical research, the consequences in biology and medicine, and the possible solutions for obtaining the actual amino acid sequence of proteins in the post-genomics era. Among the known human genes whose study was affected by this artifact, we can include disco interacting protein 2 homolog A (DIP2A KIAA0184), Down syndrome critical region 1 (DSCR1), SON DNA binding protein (SON), trefoil factor 3 (TFF3) and URB1 ribosome biogenesis 1 homolog (URB1 KIAA0539) on chromosome 21, as well as receptor for activated C kinase 1 (RACK1, also known as GNB2L1), glutaminyl‑tRNA synthetase (QARS) and tyrosyl-DNA phosphodiesterase 2 (TDP2) along with another 474 loci, including interleukin 16 (IL16). The known difficulty in obtaining the actual full length, complete sequence of a messenger RNA (mRNA) may lead to the erroneous determination of its coding sequence at the 5' region (5' end mRNA artifact), and consequently to the wrong assignment of the translation start codon, leading to the inaccurate prediction of the encoded polypeptide at its amino terminus.
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